ISC Biotechnology 2011 Class-12 Previous Year Question Papers Solved

ISC Biotechnology 2011 Class-12 Previous Year Question Paper Solved for practice. Step by step Solutions with Questions of Part-1 and Part-2 (Section A , Section-B). By the practice of Biotechnology 2011 Class-12 Solved Previous Year Question Paper you can get the idea of solving.

Try Also other year except ISC Biotechnology 2011 Class-12 Solved Question Paper of Previous  Year for more practice. Because only ISC Biotechnology 2011 Class-12 is not enough for complete preparation of next council exam. Visit official website CISCE for detail information about ISC Class-11 Biotechnology.

ISC Biotechnology 2011 Class-12 Previous Year Question Paper Solved


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Part-I

 Part-II


Maximum Marks: 80
Time allowed: Three hours

  • Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.
  • Answer Question 1 (Compulsory) from Part I and five questions from Part II, choosing two questions from Section A, two questions from Section B and one question from either Section A or Section B.
  • The intended marks for questions or parts of questions are given in brackets [ ].
  • Transactions should be recorded in the answer book.
  • All calculations should be shown clearly.
  • All working, including rough work, should be done on the same page as, and adjacent to the rest of the answer.

Part -1 (20 Marks)
(Answer all questions)

ISC Biotechnology 2011 Class-12 Previous Year Question Paper Solved 

Question 1
(a) Mention any one significant difference between each of the following : [5] (i) Nucleotide and nucleoside.
(ii) Plasmids and phages.
(iii) Fluorescence spectrometry and mass spectrometry.
(iv) Primer and primases.
(v) Introns and exons.

(b) Answer the following questions : [5]
(i) What are oil eating bacteria?
(ii) What are single cell proteins?
(iii) Who proposed the Operon concept of gene regulation?
(iv) Explain the term transposons.
(v) Name one polysaccharide found in the cell wall of bacteria and one polysaccharide found in fungi.

(c) Write the full form of the following: [5]
(i) NCBI
(ii) EST
(iii) ROM
(iv) PACE
(v) FMN

(d) Explain briefly: [5]
(i) RNA dependent DNA polymerase
(ii) EMBL
(iii) Isoelectric focussing
(iv) Cosmids
(v) Vitamins
Answer 1:
(a)

 (i) Nucleotide: The nucleotide is a condensation product of heterocyclic nitrogen base, a pentose sugar like ribose or deoxyribose, and a phosphate or polyphosphate group.

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Nucleoside: Nucleoside consisting of a pentose sugar, usually ribose or deoxyribose, and a nitrogen base-purine or pyrimidine.

(ii) Plasmids: Plasmids are the extra-chromosomal, independent, self replicating, circular, double stranded DNA molecules naturally found in all bacteria and some fungi.

Phages: Bacteriophage (i.e., viruses that infect bacteria) are obligate intracellular parasites that multiply inside bacteria by making use of some or all of the host biosynthetic machinery.

(iii) Fluorescence Spectrometry: Fluorescence spectrophotometry is a technique that assay the state of a biological system by studying the absorption spectra of different radiations and its interactions with fluorescent probe molecules.

Mass Spectrometry: Mass spectrometry (MS) is an analytical technique that involves separation of ions of different mass and energy when fixed magnetic and electric fields are employed.

(iv) Primer: A primer is a short strand of RNA that serves as a starting point for DNA synthesis They are required for DNA replication because DNA polymerases can only add new nucleotides to an existing strand of DNA.

Primases: Primase is an enzyme that catalyzes the synthesis of a short RNA segment (called a primer) complementary to a ssDNA template.

(v) Introns: Introns are non-coding DNA base sequences , which are found between exons, but are not transcribed part of mature mRNA.

Exons: Exons are coding DNA base sequences that are transcribed into wzRNA and finally code for amino acids in the proteins.

(b) (i) Bacteria that decompose and use oil as their source of energy are called as oil eating bacteria e.g., Pseudomonasputida, P.capacia etc. are efficient degraders created through genetic engineering. They need to be established in environment. They (“the oil-eating bacteria”) could digest about two- thirds of the hydrocarbons that would be found in a typical oil spill.

(ii) The term single cell protein (SCP) refers to biomass of dry cells of microorganisms such as yeast, bacteria, mushroom (fungi) and algae which grow on different carbon sources. The name “single cell protein” was used for the first time by the M.I.T. Professor Carol Wilson.

(iii) Jacob and Monod proposed the Operon concept of Gene Expression in bacteria.

(iv) Transposons : Transposons are sequences of DNA that can move around to different positions within the genome of a single cell. Transposons are called “Jumping genes”, and are examples of mobile genetic elements.
Two types of Transposons are possible :

  • Retrotrahsposons and
  • DNA transposons.

In this process, they may :

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  • cause mutations
  • increase (or decrease) the amount of DNA in the genome.

(v) Peptidoglycans (mucopeptides, glycopeptides, mureins) are the structural elements of bacterial cell walls. Fungi possess cell walls made-up of chitin, polymer of glucosamine,

(c)

(i) NCBI: National Centre for Biotechnology Information

(ii) EST : Expressed sequenced tag

(iii) ROM : Read only memory

(iv) PAGE : Polyacrylamide gel electrophoresis

(v) FMN : Flavin mononucleotides.

(d) (i) RNA-dependent DNA polymerase enzyme is used for the synthesis of a complementary DNA strand from RNA molecule as a template. It is produced by HTV and other retroviruses which help them to synthesize DNA from their viral RNA.

(iii) Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by their electric charge differences. It is a type of zone electrophoresis, usually performed on proteins in a gel.

(iv) Cosmids are constructed by uniting some part of the bacterial plasmids with a ‘ori’ gene, an antibiotic selection marker and a cloning site with one or more recently two ‘cos’ sites derived from bacteriophage lambda.

(v) A vitamin is an organic compound required as a nutrient in very small amounts by an organism. They act as co-factor of an enzyme. It cannot be synthesized in sufficient quantities by an organism, and must be obtained from the diet.


Part – II (50 Marks)
(Answer any Five questions)

ISC Biotechnology 2011 Class-12 Previous Year Question Paper Solved 

Question 2.
(a) Mention two important chemical properties of each of the following: [4] (i) Monosaccharides
(ii) Proteins .
(b) What are restriction enzymes ? How do thev act ? Give any two examples of restriction enzymes. [4] (c) What are derived lipids ? Give an example. [2] Answer 2:
(a)

 (i) Chemical properties of monosaccharides :
When a & p isomeric forms of D-glucose are dissolved in water. Their optical rotation changes with time and approaches the final equilibrium value. +53°. This change is known as mutarotation. Mutarotation occurs because of slow conversion of α -D glucose and β-D glucose via open chain form until equilibrium is established giving a constant rotation +53°.

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