ISC Biotechnology 2014 Class-12 Previous Year Question Papers Solved

ISC Biotechnology 2014 Class-12 Previous Year Question Paper Solved for practice. Step by step Solutions with Questions of Part-1 and Part-2 (Section A , Section-B). By the practice of Biotechnology 2014 Class-12 Solved Previous Year Question Paper you can get the idea of solving.

Try Also other year except ISC Biotechnology 2014 Class-12 Solved Question Paper of Previous  Year for more practice. Because only ISC Biotechnology 2014 Class-12 is not enough for complete preparation of next council exam. Visit official website CISCE for detail information about ISC Class-12 Biotechnology.

ISC Biotechnology 2014 Class-12 Previous Year Question Paper Solved

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Maximum Marks: 80
Time allowed: Three hours

  • Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.
  • Answer Question 1 (Compulsory) from Part I and five questions from Part II, choosing two questions from Section A, two questions from Section B and one question from either Section A or Section B.
  • The intended marks for questions or parts of questions are given in brackets [ ].
  • Transactions should be recorded in the answer book.
  • All calculations should be shown clearly.
  • All working, including rough work, should be done on the same page as, and adjacent to the rest of the answer.

Part -1 (20 Marks)
(Answer all questions)

ISC Biotechnology 2014 Class-12 Previous Year Question Paper Solved

Question 1.
(a) Mention any one significant difference between each of the following : [5] (i) RNA polymerases and Tag DNA polymerases
(ii) ln-situ conservation and Ex-situ conservation
(iii) Micronutrients and Macronutrients
(iv) mRNA and tRNA
(v) Essential amino acids and Non-essential amino acids

(b) Answer the following questions : [5]
(i) Why is primer essential during DNA replication ?
(ii) Name a pest resistant crop developed using biotechnology techniques.
(iii) Why are glucose and lactose referred to as reducing sugars ?
(iv) What is the role of dcMTP during DNA sequencing ?
(v) How is the disease alkaptonuria caused ?

(c) Write the Ml form of the following: [5] (i) BLAST
(ii) ESTS
(iii) MOD
(iv) RAM
(v) SNP

(d) Explain briefly: [5]
(i) Splicing
(ii) Mitotic arrest
(iii) Reverse transcription
(iv) Interferon
(v) Activation energy of enzymes
Answer 1:

(i)RNA Polymerase: It is an enzyme found in both prokaryotic and eukaryotic cells. It catalyzes the transcription of DNA to synthesize precursors of tmRNA.

Taq DNA Polymerase: It is a thermostable DNA polymerase, originally isolated from bacterium, Thermus aquaticus. It is used in polymerase chain reaction.

(ii) In-situ conservation: It means on-site conservation. It is the process of protecting an endangered plant or animal species in its natural habitat either by protecting or cleaning up the habitat itself, or by defending the species from predators.

Ex-situ conservation: It means literally, “off-site conservation”. It is the process of protecting an endangered species of plant or animal by removing part of the population from a threatened habitat and placing it in a new location, which may be a wild area or within the care of humans e.g., Zoos, Botanical garden, etc.

(iii) Micronutrients: Micronutrients are essential elements required by organisms in small quantities. They include microminerals and vitamins.

Macronutrients: Macronutrients include carbohydrates, proteins and fats required in larger quantities than micronutrients in the body

(iv) mRNA: mRNA is present in nucleus and functions in cytoplasm. It carries messages from DNA.

tRNA: tRNA is an adapter present in cytoplasm. It recognizes and brings amino acids near ribosomes for protein synthesis.

(v) Essential amino acid: The essential amino acids cannot be synthesized by the human body and are obtained from food.

Non-essential amino acid: The non-essential amino acids can be synthesized by the human body. They can be produced from other amino acids and substances in the diet and metabolism.


(i) A primer is a strand of short RNA that functions as a starting point for DNA replication. It is required because the enzymes DNA polymerases that catalyze replication, can only add new nucleotides to an existing strand of DNA.

(ii) Bt crop is a pest resistant crop developed by using biotechnology techniques.

(iii) Glucose and lactose are the reducing sugars as they have an open chain with a free aldehyde or a ketone group.

(iv) ddNTP’s are a form of nucleotide that inhibit extension of the primer in DNA sequencing. Once a ddNTP has been incorporated into the DNA chain, it halts the process.

(v) Alkaptonuria (black mine disease or alcaptonuria): It is a rare, inherited recessive genetic disorder of tyrosine metabolism, caused due to mutation of gene.
Symptoms : Black urine, ochronosis, leading to osteoarthritis, kidney stones.

(c) (i) BLAST : Basic Local Alignment Search Tool

(ii) EST : Expressed Sequence Tag

(iii) MGD : Mouse Genome Database

(iv) RAM : Random Access Memory

(v) SNP : Single Nucleotide Polymorphism.


(i) Splicing is the process by which introns(Non-coding) are removed from heteronuclear RNA to produce mature functional messanger RNA by uniting exons.

(ii) The process by which the mitotic cell cycle is stopped during one of the normal phases G1S, G2,M) of cell cycle.

(iii) It is the process by which DNA is synthesized from RNA template by reverse transcriptase enzyme. This process may be particularly helpful in treating diseases like HIV.

(iv) Interferon is a protein released by animal cells, generally in response to the presence of a virus. It inhibits virus replication.

(v) Activation energy of enzyme is the amount of energy required to bring substrates together to the point where they can react.

Part – II (50 Marks)
(Answer any Five questions)

ISC Biotechnology 2014 Class-12 Previous Year Question Paper Solved 

Question 2.
(a) What are polysaccharides ? Name any three naturally occurring polysaccharides and give their structural units. [4] (b) State one important use of each of the following in biotechnology : [4] (i) Genomic DNA library and cDNA library
(ii) Transfection and Transformation
(c) What are single cell proteins ? [2] Answer 2:
(a) Polysaccharides are the complex polymeric carbohydrate molecules composed of long chains of monosaccharide units linked together by glycosidic bonds. They vary in structure from linear to highly branched. Examples include storage polysaccharides such as starch and glycogen, and structural polysaccharides such as cellulose and chitin. Starch, glycogen and cellulose are the natural polysaccharide and their structural units are glucose.


(i) Genomic Library: This is a collection of clones that represent the complete genome of an organism. For construction of a Genomic library the entire genomic DNA is isolated from host cells or tissues, purified and broken randomly into fragments of correct size for cloning into a suitable vector.

The major use of Genomic library is hierarchichal shotgun sequencing.
cDNA Library : The library made from complementary or copy DNA (cDNA) is called cDNA library. The library represents the DNA of only eukaryotic organisms, not the prokaryotic once.

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