ISC Biotechnology 2019 Class-12 Previous Year Question Papers Solved

ISC Biotechnology 2019 Class-12 Previous Year Question Paper Solved for practice. Step by step Solutions with Questions of Part-1 and Part-2 (Section A , Section-B). By the practice of Biotechnology 2019 Class-12 Solved Previous Year Question Paper you can get the idea of solving.

Try Also other year except ISC Biotechnology 2019 Class-12 Solved Question Paper of Previous  Year for more practice. Because only ISC Biotechnology 2019 Class-12 is not enough for complete preparation of next council exam. Visit official website CISCE for detail information about ISC Class-12 Biotechnology.

ISC Biotechnology 2019 Class-12 Previous Year Question Paper Solved


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Part-I

 Part-II


Maximum Marks: 80
Time allowed: Three hours

  • Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time.
  • Answer Question 1 (Compulsory) from Part I and five questions from Part II, choosing two questions from Section A, two questions from Section B and one question from either Section A or Section B.
  • The intended marks for questions or parts of questions are given in brackets [ ].
  • Transactions should be recorded in the answer book.
  • All calculations should be shown clearly.
  • All working, including rough work, should be done on the same page as, and adjacent to the rest of the answer.

Part -1 (20 Marks)
(Answer all questions)

ISC Biotechnology 2019 Class-12 Previous Year Question Paper Solved 

Question 1.
(a) Mention any one significant difference between each of the following : [5] (i) Anti codon and codon
(ii) Intrinsic fluorescence and extrinsic fluorescence
(iii) Introns and Exons
(iv) Genomic DNA library and cDNA library
(v) RAM and ROM

(b) Answer the following questions: [5] (i) Which amino acid is optically inactive and why ?
(ii) What is meant by exponential phase 9
(iii) What are designer oils ?
(iv) What is palindromic sequence 9
(v) Which substance is used in diploidization of haploid plants ?

(c) Write the full form of each of the following : [5] (i) NBRI
(ii) NBTB
(iii) BLAST
(iv) PIR
(v) YAC

(d) Explain briefly the following terms : [5] (i) Callus
(ii) SNPs
(iii) Lyophilisation
(iv) Gene cloning
(v) Cybrids
Answers 1: (ISC Biotechnology 2019 Class-12)
(a) difference between Anti codon and codon

(i) Anti codon is the corresponding triplet sequence on the transfer RNA (tRNA) which brings in the specific amino acid to the ribosome during translation. The anti codon is complementary to the codon, that is, if the codon is AUU, then the anti codon is UAA.
Codon is the triplet sequence in the messenger RNA (mRNA) transcript which specifies a corresponding amino acid (or a start or stop command).

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(ii) difference between Intrinsic fluorescence and extrinsic fluorescence

Intrinsic fluorescence Extrinsic fluorescence
The fluorescence shown by natural compounds is known as intrinsic fluorescence. There are certain chemical compounds which do not fluorescence. It can be detected after coupling them with a fluorescence probe or flour. This phenomenon is called extrinsic fluorescence.

 

(iii) difference between Introns and Exons

Introns Exons
Introns are non-coding DNA base sequences, which are found between exons, but are not transcribed part of mature mRNA. Exons are coding DNA base sequences that are transcribed into w/RNA and finally code for amino acids in the proteins.

 

(iv)  difference between Genomic DNA Library and cDNA Library

Genomic DNA Library cDNA Library
(1) It is a collection of clones that represent the complete genome of an organism.

(2) It is the mixture of genomic DNA.

(1) The library made from complementary or copy DNA (cDNA) is called cDNA library.

(2) cDNA library consists of cDNA clones prepared by using mRNA.

 

(v) difference between RAM and ROM

RAM ROM
(1) It stores data and programme temporarily.

(2) It contains the data of operating system, software’s and other application programmes.

(1) It provides a non-volatile storage of data.

(2) It loads operating system into the memory and starts the computer when switched on.

 

(b)

(i) Glycine is the only amino acid which is optically inactive. It is the first neutral amino acid. In Glycine, the central atom has 2 hydrogen atoms. It does not contain any chiral carbon that’s why it is optically inactive.
ISC Biotechnology Question Paper 2019 Solved for Class 12 1

(ii) Exponential Phase : It is a growth phase. In the exponential (log) phase, cells divide as fast as possible according to the growth medium, the microorganism itself and environmental conditions. This phase has a limited duration.
ISC Biotechnology Question Paper 2019 Solved for Class 12 2
(iii) Designer Oil : “Designer oil” that reduces LDL ( bad ) blood cholesterol levels in humans and increases energy expenditure, which may prevent people from gaining weight. The oil incorporates a phytosterol-based functional food ingredient Phytrol (TM) from Forbes into oil using proprietary technology.

(iv) Palindromic sequence are the base pair sequences that are the same when read forward (left to right) or backward (right to left) from a central axis of symmetry. The sequences read the same on the two strands in 5 → 3 direction and this is also true when we read in the 3 → 5 directions.

(v) Colchicine is used in diploidization of haploid plants. There are mainly two approaches for diploidization-colchicine treatment and endometriosis.

(c) NBRI: National Botanical Research Institute
NBTB : National Biotechnology Board
BLAST: Basic Local Alignment Search Tool
PIR : Protein Information Resource
YAC : Yeast Artificial Chromosome

(d)

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(i) Callus : It is a mass of meristematic undifferentiated unorganized cells produced in a culture.

(ii) SNPs : Single Nucleotide Polymorphisms (SNPs) are the variations in a nucleotide at genomic DNA in different individuals at a population which occur due to change even in a single base (e.g., A, G, T or C). In human genome, SNPs occur at 1 n – 3.2 million sites.

(iii) Lyophilisation : In this method the microbial suspension is put in small ampoules and frozen through drying under vacuum. Vaccum drying results in sublimation of cell water. The lyophilised cultures are stored at 4°C. The culture remain viable for about 10 years. By this method a large number of cultures are maintained without variation in the characteristics.

(iv) Gene Cloning : It is a process of copying a specific desired gene/section on DNA and producing in large number/quantities.

(v) Cybrids : A cytoplasmicallv hybrid cell with organelles from both parental sources (obtained through fusion of cytoplast with a whole cell) and a nucleus of only one cell Nucleus of the other cell denatured.


Part – II (50 Marks)
(Answer any Five questions)

ISC Biotechnology 2019 Class-12 Previous Year Question Paper Solved 

Question 2.
(a) With reference to composition of culture medium, answer the following : [4] (i) Cytokinins
(ii) Auxin’s
(b) Explain the induced fit hypothesis of enzyme action with the help of suitable illustrations. [4] (c) Write a note on Quaternary’ structure of proteins. [2] Answers 3:  (ISC Biotechnology 2019 Class-12)
(a)

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